Watcher 1.0 including CaMV35S, NOS and plant internal gene

Technology Platform: Gel-based PCR

Size: 75 Reactions; 100 Reactions; 150 Reactions; 300 Reactions

Kit Storage: -20°C

Applications

For qualitative detection of 35S promoter and NOS terminator genetic sequences. Most transgenes are inserted between the 35S promoter sequence isolated from Cauliflower Mosaic Virus and the nopaline synthase terminator sequence isolated from Agrobacterium tumefaciens. Detection of these promoter/terminator sequences provides a general screening of genetic modification. The U-Plant marker is for detection of the presence of plant specific genetic sequences.

Kit Components

35S Mastermix (2 x 275 μl)
NOS Mastermix (2 x 275 μl)
U-Plant Mastermix (2 x 275 μl)
Positive Control (1 x 90 μl)
Taq Polymerase (1 x 30 μl)

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