Hunter 1.0 including CaMV35S, NOS and plant internal gene

Technology Platform: Real-time PCR

Size: 75 Reactions; 100 Reactions; 150 Reactions; 300 Reactions

Kit Storage: -20°C

Application

For qualitative detection of 35S promoter and NOS terminator genetic sequences. Most transgenes are inserted between the 35S promoter sequence isolated from Cauliflower Mosaic Virus and the nopaline synthase terminator sequence isolated from Agrobacterium tumefaciens. Detection of these promoter/terminator sequences provides a general screening of genetic modification. The U-Plant marker is for detection of the presence of plant specific genetic sequence.

Machine Platform:
For use with real-time cyclers including ABI PRISM® 7000, 7700 and 7900HT; ABI 7300 and 7500; Bio-Rad iCycler™; Stratagene Mx3000P™, Mx3005P™ and Mx4000™; Corbett Research Rotor-Gene™, MJ Research DNA Engine Opticon™, Opticon®2 and Chromo 4™, Cepheid Smart Cycler®

Kit Components

35S Real-time Mastermix (3 x 400 μl)
NOS Real-time Mastermix (3 x 400 μl)
U-Plant Real-time Mastermix (3 x 400 μl)
Positive Control (1 x 90 μl)

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